Code No.: 5734                                                                                               Sub. Code: LBT1
M.PHIL. DEGREE EXAMINATION, NOVEMBER 2008
First Semester
BOTANY
Paper I
RESEARCH METHODOLOGY
(For those who joined in July 2008 and onwards)
Time: Three hours                                                                                            Maximum : 75 marks
PART A - (10 x 2 = 20 Marks)
Answer ALL questions in about 50 words each.
Write short notes on:
  1. Dehydration
  2. TEM
  3. Biosensor
  4. Radioactive decay
  5. TLC
  6. PCR
  7. Chi-Square test
  8. ANOVA
  9. Internet
  10. e-book
PART B - (5 x 6 = 30 marks)
Answer ALL questions in about 250 words each choosing either (a) or (b).
11. (a) Define the staining process.
or
(b) Define Eleromagnetic radiation
12. (a) Define Biochips and its importance
or
(b) Explain the method of labeling of biological molecules.
13. (a) Explain the types of chromatography.
or
(b) Define the importance of trans-illuminator.
14. (a) Explain Regression Analysis
or
(b) Define sampling technology in statistical studies.
15. (a) Explain the components of research paper.
or
(b) Explain the importance of reference collection.
PART C - (5 x 10 = 50 marks)
Answer ALL questions in about 500 words each choosing either (a) or (b).
16. (a) Write an essay on SEM.
or
(b) Briefly write about fluorescent microscopy.
17. (a) Explain the methods involved in the detection and measurement of radioactivity.
or
(b) Write an essay on IR
18. (a) Explain the importance of horizontal electrophoresis.
or
(b) Explain RHPD analysis in detail.
19. (a) oWrite an essay on Standard deviation and Correlation.
or
(b) Write notes on
(i) Probability normal and bionomial distribution
(ii) Measures of Dispersion.
20. (a) Discuss the importance of Internet in updating literature.
or
(b) How can you carryout the literature and reference collection and prepare it in thesis format?


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Code No.: 5735                                                                                               Sub. Code: LBT2
M.PHIL. DEGREE EXAMINATION, NOVEMBER 2008
First Semester
BOTANY
Paper II
PLANT BIOTECHNOLOGY
(For those who joined in July 2008 and onwards)
Time: Three hours                                                                                            Maximum : 75 marks
PART A - (10 x 2 = 20 Marks)
Answer ALL questions in about 50 words each.
Write short notes on:
  1. Phagemid
  2. Gene Library
  3. Molecular farming
  4. Transgenic plants
  5. Germ plasm
  6. Haploids
  7. Proteomics
  8. Functional genomics
  9. POPGENE
  10. Patent Right
PART B - (5 x 6 = 30 marks)
Answer ALL questions in about 250 words each choosing either (a) or (b).
11. (a) Explain the gene transfer methods.
or
(b) Write a short note on gene sequencing.
12. (a) Explain the mechanism of t-DNA transfer.
or
(b) Explain gene silencing with an example.
13. (a) Explain the method of biotransformation.
or
(b) How cryopreservation is useful in tissue culture studies?
14. (a) Explain gene mapping in prokaryotes.
or
(b) Define molecular marker in detail.
15. (a) Define PBR in detail.
or
(b) Explain tools of Bioinformatics.
PART C - (5 x 10 = 50 marks)
Answer ALL questions in about 500 words each choosing either (a) or (b).
16. (a) Write on the types and role of marker genes.
or
(b) Write notes on:
(i) Shuttles vector
(ii) Target genes
17. (a) Explain the structure and organization of Ti plasmid.
or
(b) Explain how tissue culture technique is useful in overcoming the stress and pest problem in plants.
18. (a) Write an essay on micro propagation.
or
(b) Explain the method of invitro production of secondary metabolites.
19. (a) Write an essay on Genomics
or
(b) Write notes on
(i) Human gene
(ii) Pattern of gene expression
20. (a) Write an essay on Plant Genomic Resources and its importance.
or
(b) Write notes on:
Sequence database searching
Phylogenetic analysis


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